About Recombinant DNA

Bioimanay has developed a recombinant protein yeast-based expression system, with capability to produce recombinant proteins.

Through recombinant DNA technology, we are able to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in biological organisms. This strategy allows to tune proteins properties to obtain particular functions or characteristics. 

DNA molecules from all organisms share the same chemical structure. They differ only in the sequence of nucleotides within that identical overall structure. Consequently, when DNA from a foreign source is linked to host sequences that can drive DNA replication and then introduced into a host organism, the foreign DNA is replicated along with the host DNA.

1. Genetic Design
This technique includes mapping and analyzing DNA and protein sequences, aligning different DNA and protein sequences to compare them and design a new DNA secuence that will be introduced into the host organism.

2. Gene Construction
The gene to be inserted into the genetically modified organism must be combined with other genetic elements in order for it to work properly. The gene can also be modified at this stage for better expression or effectiveness. As well as the gene to be inserted most constructs contain a promoter and terminator region as well as a selectable marker gene. The promoter region initiates transcription of the gene and can be used to control the location and level of gene expression, while the terminator region ends transcription.

3. Microorganism Transformation and Selection
An organism's genome is manipulated using modern DNA technology. It involves the introduction of foreign DNA or synthetic genes into the organism to be transformed.
Not all the organism's cells will be transformed with the new genetic material; in most cases a selectable marker is used to differentiate transformed from untransformed cells. If a cell has been successfully transformed with the DNA it will also contain the marker gene. By growing the cells in the presence of an antibiotic or chemical that selects or marks the cells expressing that gene it is possible to separate the transgenic events from the non-transgenic.

4. Biological Tests
Once the genetic manipulated microorganism is obtained, it is tested in the laboratory to confirm that the recombinant protein is expressed by the microorganism genome, and that the recombinant protein produces the desired biological effect (enzimatic, diagnosis, vaccines, others).

5. Process Engineering and Scaling-up
The microorganism is optimized together with the fermentation process, scaling up from small laboratory bioreactors, to pilot plant scale, and finally to industrial scale.

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